DeLisle is best known for roles such as Vicky from The Fairly Oddparents, Emily from Clifford the Big Red Dog, Mandy from The Grim Adventures of Billy and Mandy, Sam Manson from Danny Phantom, Frankie Foster from Foster's Home For Imaginary Friends, Azula from Avatar: The Last Airbender, Wubbzy from Wow! Wow! Wubbzy!, Frida Suárez from El Tigre: The Adventures of Manny Rivera, Betty Brant from The Spectacular Spider-Man, Amanda Valenciano Libre from Metal Gear Solid: Peace Walker, and some of the Loud sisters from The Loud House. She has also performed modern portrayals of Daphne Blake from Scooby-Doo, Betty Rubble from The Flintstones, Olive Oyl from Popeye, Jane Jetson from The Jetsons and Sherri, Terri and Martin Prince from The Simpsons.
DeLisle has also been heard in The Powerpuff Girls, Rugrats, The Weekenders, Codename: Kids Next Door, Evil Con Carne, Star Wars: Clone Wars, Xiaolin Showdown, Justice League Unlimited, Hi Hi Puffy AmiYumi, My Gym Partner's A Monkey, Ultimate Avengers, Batman: The Brave and the Bold, Bolt, Firebreather, Generator Rex, T.U.F.F. Puppy, The Looney Tunes Show, Adventure Time, Winx Club, Green Lantern: The Animated Series, Gravity Falls, Sanjay and Craig, Beware the Batman, The Tom and Jerry Show, When Marnie Was There, Star vs. the Forces of Evil, Mighty Magiswords, and Unikitty.
As a singer, DeLisle has also released several country music albums, starting with The Small Time in 2000.